论文部分内容阅读
AIM: To clarify the expression patterns and prognostic implications of the mitotic regulator Polo-like kinase 1 (PLKl) in colon cancer. METHODS: Expression of PLKl was investigated by immunohistochemistry (158 cases) and immunoblotting in tissue of colon adenomas and adenocarcinomas. PLKl expression patterns were correlated with clinicopathological parameters and patient prognosis. In addition, expression of PLKl was evaluated by immunoblot and PCR in colon carcinoma cell lines, and coexpression of PLKl with the proliferation marker Ki-67 was investigated. RESULTS: Weak PLKl expression was observed in normal colon mucosa and adenomas. In contrast, 66.7% of carcinomas showed strong expression of PLKl. Overexpression of PLKl correlated positively with Dukes stage (P<0.001), tumor stage (P= 0.001) and nodal status (P<0.05). Additionally, PLKl expression was a prognostic marker in univariate survival analysis (P<0.01) and had independent prognostic significance (RR = 3.3, P= 0.02) in patients with locoregional disease. Expression of PLKl mRNA and protein was detected in all cell lines investigated. Coexpression of PLKl and Ki-67 was observed in the majority of colon cancer cells, but a considerable proportion of cells showed PLKl positivity without Ki-67 expression. CONCLUSION: PLKl is a new prognostic marker for colon carcinoma patients and may be involved in tumorigenesis and progression of colon cancer. Strategies focusing on PLKl inhibition in vivo might therefore represent a promising new therapeutic approach for this tumor entity.
AIM: To clarify the expression patterns and prognostic implications of the mitotic regulator Polo-like kinase 1 (PLK1) in colon cancer. METHODS: Expression of PLK1 was investigated by immunohistochemistry (158 cases) and immunoblotting in tissue of colon adenomas and adenocarcinomas. PLK1 expression patterns were correlated with clinicopathological parameters and patient prognosis. In addition, expression of PLK1 was evaluated by immunoblot and PCR in colon carcinoma cell lines, and coexpression of PLK1 with the proliferation marker Ki-67 was investigated. RESULTS: Weak PLK1 expression was observed In normal, 66.7% of carcinomas showed strong expression of PLK1. Overexpression of PLK1 positively correlated with Dukes stage (P <0.001), tumor stage (P = 0.001) and nodal status (P <0.05). Additionally, PLK1 expression was a prognostic marker in univariate survival analysis (P <0.01) and had independent prognostic significance (RR = 3.3, P = 0.02) in patie nts with locoregional disease. Expression of PLK1 mRNA and protein was detected in all cell lines investigated. Coexpression of PLK1 and Ki-67 was observed in the majority of colon cancer cells, but a proportion of cells showed PLK1 positivity without Ki-67 expression . CONCLUSION: PLK1 is a new prognostic marker for colon carcinoma patients and may be involved in tumorigenesis and progression of colon cancer. Strategies focusing on PLK1 inhibition in vivo might therefore a promising new therapeutic approach for this tumor entity.