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本文旨在评价小肠内毒素、肿瘤坏死因子(TNF)和中性粒细胞在肠缺血再灌注损伤伴随肺损伤发病机理的关系。采用100～150g重Sprague Dawley雄鼠。麻醉、剖腹,夹住肠系膜上动脉引起肠缺血,二次剖腹解除使恢复再灌注,以激光多普勒测粘膜血流量。再灌注15～120分钟,处死动物取其肺组织和血样作下列分析测定:1)内毒素测定,2)TNF-α活性测定,3)肺微血管损伤的评估,4)髓过氧化物酶(MPO)含量,5)抗
This article aims to evaluate the relationship between intestinal endotoxin, tumor necrosis factor (TNF) and neutrophils in the pathogenesis of intestinal ischemia-reperfusion injury accompanied by lung injury. 100 ~ 150g heavy Sprague Dawley male rats were used. Anesthesia, Caesarean section, clamping the superior mesenteric artery causing intestinal ischemia, secondary Caesarean section to restore the reperfusion, laser mucosal blood flow by laser Doppler. Reperfusion 15 to 120 minutes, the animals were sacrificed to take the lung tissue and blood samples for the following analysis determination: 1) endotoxin determination, 2) TNF-α activity, 3) assessment of pulmonary microvascular injury, 4) myeloperoxidase MPO) content, 5) anti