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目的：制备人源性重组ＨＢｓＡｇ抗体Ｆａｂ片段。方法：采用固相ＨＢｓＡｇ，从已建立的人源性抗体组合文库中筛选针对ＨＢｓＡｇ的抗体子文库，并转入大肠杆菌中表达。反复冻融细菌，获得可溶性Ｆａｂ片段。制备羊抗人ＩｇＧＦａｂ抗体亲和层析柱、纯化Ｆａｂ片段。ＳＤＳ－ＰＡＧＥ及点印迹法分析鉴定纯化后Ｆａｂ片段的纯度及结合ＨＢｓＡｇ的能力。结果：蛋白印迹显示转化后的细菌内有可溶性Ｆａｂ片段的表达。纯化后的Ｆａｂ片段达免疫纯，并具有特异性结合ＨＢｓＡｇ的能力。结论：该研究为人源性抗ＨＢｓＡｇＦａｂ用于临床治疗打下基础。
Objective: To prepare Fab fragment of human recombinant HBsAg antibody. METHODS: Antibody sub-libraries against HBsAg were selected from established human antibody combinatorial libraries using solid phase HBsAg and transformed into E. coli for expression. Bacteria were freeze-thawed repeatedly to obtain soluble Fab fragments. A goat anti-human IgGFab antibody affinity column was prepared and the Fab fragment was purified. SDS-PAGE and dot blot analysis were used to identify the purity of the purified Fab fragment and its ability to bind to HBsAg. Results: Western blotting revealed the expression of soluble Fab fragments in transformed bacteria. The purified Fab fragment is immunopurified and has the ability to specifically bind to HBsAg. Conclusion: This study lays the foundation for human anti-HBsAgFab for clinical treatment.