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以细胞色素c(Cyt c)为模型蛋白,采用表面增强红外吸收光谱监测了三明治结构所吸附的纳米金对氧化还原诱导的Cyt c表面增强红外差谱的改变.研究表明,在单层Cyt c分子表面组装纳米金,使得血红素的红外差谱特征峰明显增强,这归因于纳米金和血红素之间的电子传递.纳米金与Cyt c氧化还原活性中心血红素的相互作用加速了蛋白质的电子传递.这为实现并优化表面吸附蛋白质的直接电化学提供了一种新技术.
Using cytochrome c (Cyt c) as a model protein, surface enhanced infrared absorption spectroscopy was used to monitor the changes in the infrared difference spectrum of Cyt c surface-enhanced redox induced by the sandwich gold nanoparticles adsorbed by the sandwich structure. The nanometer gold is assembled on the surface of the molecule, so that the characteristic peak of the infrared difference spectrum of heme is obviously enhanced, which is attributed to the electron transfer between the nano-gold and the heme.The interaction between the nano-gold and the heme of the Cyt c redox active center accelerates the protein This provides a new technique for achieving and optimizing the direct electrochemistry of surface-adsorbed proteins.