去大脑僵直是重要的神经生理实验,由于传统的方法需要开颅手术,操作难度大,不容易掌握,而且极易造成家免出血过快过多,导致实验动物很快死亡,所以实验成功率受到明显影响。对此我们总结出了颅外穿刺横断中脑法新的实验技术,解决了上述问题,经过几年的尝试,取得了令人满意的效果。现报告如下。1 手术和画线:选取体重在1.5kg～2.0kg 的家兔为实验动物,对家兔不麻醉(也可为了起到镇静作用 To the brain stiffness is an important neurophysiological experiment, due to the traditional method requires craniotomy, the operation is difficult, not easy to grasp, and can easily cause excessive bleeding at home too much, leading to rapid death of experimental animals, so the experimental success rate Affected significantly. In this regard, we summarize the new experimental technique of transcranial middle cerebral artery puncture, to solve the above problems, after several years of attempts, and achieved satisfactory results. The report is as follows. 1 surgery and drawing line: Select the body weight of 1.5kg ~ 2.0kg of rabbits as experimental animals, rabbits are not anesthetized (also can be used to play a sedative effect