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目的:探讨Lewis肺癌模型小鼠脾脏组织中CD4+CD25+调节性T细胞和TGF-β1的表达水平与肺癌发生的关系。方法:采用流式细胞仪检测CD4+CD25+调节性T细胞的比例,ELISA法检测TGF-β1的表达水平。结果:荷瘤小鼠CD4+CD25+调节性T细胞表达水平为(21.91±12.34)%,高于对照组的(14.39±4.51)%(P<0.05),荷瘤小鼠TGF-β1表达水平(3.39±1.62)μg/L,高于对照组的(2.47±0.35)μg/L(P<0.05)。结论:荷瘤小鼠CD4+CD25+调节性T细胞比例和TGF-β1表达水平增高,提示二者可能参与Lewis肺癌的发生。
Objective: To investigate the relationship between the expression of CD4 + CD25 + regulatory T cells and TGF-β1 in spleen of Lewis lung carcinoma model mice and the occurrence of lung cancer. Methods: The proportion of CD4 + CD25 + regulatory T cells was detected by flow cytometry. The expression of TGF-β1 was detected by ELISA. Results: The expression level of CD4 + CD25 + regulatory T cells in tumor-bearing mice was (21.91 ± 12.34)%, higher than that in control group (14.39 ± 4.51)% (P <0.05) 3.39 ± 1.62μg / L, higher than that of the control group (2.47 ± 0.35μg / L, P <0.05). Conclusion: The proportion of CD4 + CD25 + regulatory T cells and the expression of TGF-β1 in tumor-bearing mice are increased, suggesting that they may be involved in the development of Lewis lung cancer.