论文部分内容阅读
目的探讨羧甲基茯苓多糖(carboxymethytl pachymaran,CMP)对人外周血源性树突状细胞(dendritic cells,DC)迁移功能的影响。方法人外周血单核细胞经rhGM-CSF和IL-4诱导分化为不成熟DC,LPS诱导成熟的基础上分别加入不同质量浓度的CMP(终质量浓度为10、50、100 mg/L),以不加CMP为对照组,采用transwell小室检测DC向MIP-3迁移能力,流式细胞仪及Q-PCR检测CCR7蛋白和基因的表达情况,ELISA测定收集的上清液中IL-10的质量浓度。结果经CMP处理后DC迁移指数和CCR7的表达均升高,而上清液中IL-10的质量浓度降低,且呈剂量依赖趋势。100 mg/L CMP处理组细胞迁移指数显著高于对照组,差异有统计学意义(P<0.01)。50 mg/L和100 mg/L CMP处理组CCR7的表达显著高于对照组,而上清液中IL-10的质量浓度明显低于对照组,差异均有统计学意义(P<0.05)。结论 CMP能上调人外周血源性树突状细胞CCR7的表达并减少IL-10的分泌,增强其迁移能力。
Objective To investigate the effect of carboxymethytl pachymaran (CMP) on the migration of human peripheral blood dendritic cells (DCs). Methods Human peripheral blood mononuclear cells were induced to differentiate into immature DCs induced by rhGM-CSF and IL-4, and different concentrations of CMP (final concentration of 10, 50 and 100 mg / L) The migration of DCs to MIP-3 was detected by transwell chamber. The expression of CCR7 protein and gene was detected by flow cytometry and Q-PCR. The levels of IL-10 in supernatant were measured by ELISA concentration. Results The migration index and the expression of CCR7 increased after CMP treatment, but the concentration of IL-10 in the supernatant decreased in a dose-dependent manner. The cell migration index of 100 mg / L CMP treatment group was significantly higher than that of control group (P <0.01). The expression of CCR7 in 50 mg / L and 100 mg / L CMP treatment groups was significantly higher than that in control group, while the concentration of IL-10 in supernatant was significantly lower than that in control group (P <0.05). Conclusion CMP can up-regulate the expression of CCR7 in human peripheral blood-derived dendritic cells and decrease the secretion of IL-10 and enhance its migration ability.