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目的分级建立大鼠不同程度脊髓损伤模型。方法 140只大鼠均分为四组,采用自行研制的一种新型电控大鼠脊髓损伤打击器,以恒定的重物和打击时间,观察打击高度为1.5、2.5和6cm造成脊髓损伤后的后肢运动功能、电生理和病理变化。结果假手术组苏醒后能站立行走,CSEP-P波潜伏期恒定,脊髓结构正常。打击高度为1.5cm时(B组),动物出现轻度瘫换,28d后能站立行走,CSEP-P波延长,脊髓结构破坏较轻;打击高度为2.5cm时(C组),动物出现不全瘫,28d后不能站立行走,双后肢不能支持负重,CSEP-P波显著延长,脊髓结构破坏明显;打击高度为6.0cm时(D组),动物出现全瘫,28d后双后肢无运动,CSEP-P波始终呈直线,脊髓结构破坏严重。结论自行研制的脊髓损伤打击仪能够实现定时、定点、定高打击,可以分级、稳定的复制出轻、中、重度的脊髓损伤模型。
Objective To establish a rat model of spinal cord injury at different degrees. Methods A total of 140 rats were randomly divided into four groups. A new type of rat spinal cord injury controller was developed by ourselves. With a constant weight and time of attack, the rats were observed after the spinal cord injury at a height of 1.5, 2.5 and 6 cm Hindlimb motor function, electrophysiological and pathological changes. Results The sham-operated group was able to walk and walk after awakening. The CSEP-P wave had a constant incubation period and a normal spinal cord structure. When the height of attack was 1.5 cm (group B), the animals showed slight paralysis and became able to walk after 28 days. The CSEP-P wave prolonged and the structure of the spinal cord was damaged less. When the height of attack was 2.5 cm (group C) Paralysis, 28d after walking can not stand, double hind limbs can not support the load, CSEP-P wave was significantly prolonged, damage to the spinal structure was obvious; hit height of 6.0cm (D group), animals paralyzed, 28d after hindlimb no movement, CSEP -P wave is always straight, severely damaged spinal structure. Conclusion The SCI instrument developed by ourselves can achieve regular, fixed-point and high-impact blows, and it can classify and stably replicate the mild, moderate and severe spinal cord injury models.