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目的:探讨牙髓卟啉单胞菌(P.e)脂多糖(LPS)对小鼠成骨细胞株MC3T3-E1细胞CD14表达的影响。方法:在有血清和无血清存在的情况下,用10μg/mLP.e-LPS作用于MC3T3-E1细胞24h,流式细胞术检测CD14的表达;10μg/mLP.e-LPS刺激MC3T3-E1细胞后,观察不同时间(0、1、3、6、12及24h)后检测细胞膜结合型CD14mRNA表达的变化。应用SPSS13.0软件包对结果进行两样本t检验、单因素方差分析和Dunnettt检验。结果:流式细胞术分析P.e-LPS作用24h后,CD14蛋白表达量增加,但无血清组增加更明显;随着P.e-LPS作用时间的增加,MC3T3-E1细胞膜结合型CD14mRNA的表达量逐渐增加,作用3h时表达最明显,作用大于6h后表达量逐渐降低。结论:P.e-LPS可诱导成骨细胞MC3T3-E1的CD14表达增强,表明P.e-LPS可能通过CD14对成骨细胞发挥作用。
Objective: To investigate the effect of P.e lipopolysaccharide (LPS) on CD14 expression in mouse osteoblastic cell line MC3T3-E1. Methods: MC3T3-E1 cells were treated with 10μg / mL P.e-LPS for 24 hours in the presence of serum and serum free medium, and the expression of CD14 was detected by flow cytometry. MC3T3-E1 cells were stimulated with 10μg / mL P.e- After that, the changes of membrane-bound CD14 mRNA expression were observed after different time (0, 1, 3, 6, 12 and 24 hours) SPSS 13.0 software package was used for two-sample t test, one-way ANOVA and Dunnettt's test. Results: After treated with Pe-LPS for 24 h, the expression of CD14 protein increased, but the increase in serum-free group was more obvious. The expression of membrane-associated CD14 mRNA in MC3T3-E1 cells gradually increased with the increase of Pe-LPS , The role of the most obvious expression 3h, the role of greater than 6h after the expression decreased. CONCLUSION: P.e-LPS can induce the enhanced expression of CD14 on osteoblast MC3T3-E1, indicating that P.e-LPS may play an important role on osteoblasts through CD14.