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为了解磷细菌肥对采煤塌陷区复垦土壤放线菌群落的影响,采用Illumina高通量测序的方法,对土壤复垦5年后的放线菌群落结构及多样性进行分析,并结合冗余分析(RDA)研究放线菌群落与土壤化学性质的相关关系.结果表明:有机肥+磷细菌肥处理可以提高复垦土壤放线菌的数量.采煤塌陷复垦土壤中放线菌的优势菌群是链霉菌属(Streptomyces)和假诺卡氏菌属(Pseudonocardia),其丰度范围分别为12.28%-15.21%、8.93%-11.49%.配施磷细菌肥处理可以降低链霉菌属的相对丰度,提高假诺卡氏菌属的相对丰度.有机肥+磷细菌肥处理能够提高放线菌的Shannon-Wiener和Simpson多样性指数,对Pielou均匀度指数影响较小.RDA结果显示,土壤有机质、有效磷、速效钾、碱解氮是造成复垦土壤放线菌群落丰度和多样性差异的主要原因.综上认为,有机肥+磷细菌肥处理对复垦土壤放线菌群落的提高作用明显,可以在一定程度上加快土壤的熟化进程.
In order to understand the effect of phosphate bacteria fertilizer on actinomycete community in reclaimed soil in subsidence area of coal mining, the structure and diversity of actinomycetes community after 5 years of reclamation were analyzed by using Illumina high-throughput sequencing method Redundancy analysis (RDA) was used to study the correlation between actinomycete community and soil chemical properties.The results showed that the amount of actinomycetes in reclaimed soil could be increased by organic fertilizer + The dominant bacteria were Streptomyces and Pseudonocardia with the abundance range of 12.28% -15.21% and 8.93% -11.49% And relative abundance of Pseudonocardia increased.The organic fertilizer + Phosphobacteria treatment could improve the Shannon-Wiener and Simpson index of actinomycetes, but had little effect on Pielou evenness index.RDA The results showed that soil organic matter, available phosphorus, available potassium and alkaline hydrolysis of nitrogen were the main reasons for the difference in abundance and diversity of actinomycetes in reclaimed soil.In summary, organic manure + Actinomycetes community to improve the role of obvious, you can To a certain extent, accelerate the process of soil maturation.