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采用室内模拟盐、旱胁迫,结合田间实际测定的方法,对基因枪法获得的转BADH基因小麦多个株系的不同世代材料进行了耐盐、耐旱性鉴定。结果表明,在旱、盐胁迫条件下,转BADH基因小麦在种子萌发、幼苗生长、根系发育以及质膜保护等方面均比受体品种(对照)具有明显的优势;在田间生长条件下的转基因株系,其叶片蒸腾强度比对照明显降低,而离体叶片失水速率与对照没有明显差别。
Salt-tolerance and drought tolerance of different generations of wheat lines transformed with BADH gene obtained by particle bombardment were studied by indoor simulated salt and drought stress combined with field measurements. The results showed that BADH transgenic wheat had obvious advantages over recipient varieties (control) in seed germination, seedling growth, root system development and plasma membrane protection under drought and salt stress. Under the conditions of field growth, transgenic Compared with the control, the transpiration intensity of the leaves was significantly lower than that of the control, while the rate of water loss from the leaves was not significantly different from that of the control.