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目的检测高危型HPV感染宫颈上皮瘤样病变及宫颈癌患者外周血IL-18的水平变化,分析Th1细胞的细胞因子免疫活动,为肿瘤的免疫治疗提供实验依据。方法在细胞因子中加入荧光标记的特异性抗细胞因子单克隆抗体,特异性抗原抗体结合,以流式细胞仪分析特异性细胞因子IL-18表达水平。结果高危型HPV感染宫颈上皮瘤样病变及宫颈癌患者IL-18表达水平较正常对照组降低,差异有统计学意义。结论高危型HPV感染宫颈上皮瘤样病变及宫颈癌宫颈癌患者IL-18表达降低,这可能是肿瘤细胞发生免疫逃逸,从而导致肿瘤的发生或者转移的原因之一。
Objective To detect the level of IL-18 in peripheral blood of patients with high-risk HPV infection and cervical epithelial neoplasia, and to analyze the cytokine immune activity of Th1 cells to provide experimental evidence for the immunotherapy of tumors. Methods Cytokines were added fluorescently labeled specific anti-cytokine monoclonal antibody, specific antigen-antibody binding, flow cytometry analysis of specific cytokines IL-18 expression levels. Results High-risk HPV infection of cervical epithelial tumor-like lesions and cervical cancer patients with IL-18 expression levels lower than the normal control group, the difference was statistically significant. Conclusion High-risk HPV infection of cervical epithelial tumor-like lesions and cervical cancer cervical cancer patients with decreased expression of IL-18, which may be tumor cells immune escape, which led to the occurrence of tumor or metastasis of one of the reasons.