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目的:探讨盐酸川芎嗪联合顺铂(TMP+ DDP)对小鼠Lewis肺癌细胞侵袭转移的影响及可能的分子机制.方法:体外培养绿色荧光蛋白(GFP)标记的小鼠Lewis肺癌细胞株(LLC-GFP),盐酸川芎嗪和顺铂处理后,分别采用CCK-8法检测细胞增殖抑制率,细胞黏附实验、Transwell小室实验检测细胞侵袭、黏附能力的改变,Wes卜em Blot检测E-钙黏蛋白(E-cadherin)的表达变化.结果:与对照组相比,盐酸川芎嗪呈剂量依赖性抑制小鼠Lewis肺癌细胞的增殖;Transwell实验、细胞黏附实验显示,盐酸川芎嗪联合顺铂作用细胞24h后,与对照组相比,细胞的侵袭、黏附能力均有不同程度的下降,其抑制率呈剂量效应(P ＜0.01);Western Blot实验表明,盐酸川芎嗪联合顺铂作用细胞24h后,与对照组相比,LLC-GFP细胞中E-cadherin蛋白表达水平明显上调(P＜0.01).结论:盐酸川芎嗪联合顺铂可显著地抑制LLC-GFP细胞的生长、侵袭、黏附能力,其机制可能与其能上调E-cadherin蛋白表达水平相关.“,”Objective:To investigate the effect and mechanism of TMP and DDP on the proliferation,invasion and metastasis of mouse Lewis Lung Cancer cells in vitro.Methods:To culture LLC-GFP cells in vitro,detect cell proliferation inhibition rate in CCK-8.Transwell assay and cell adhesion test were used to determine the inhibitory effects of TMP and DDP on cell invasion and adhesion in LLC-GFP cells.Western blot assay were used to observe the expressions of E-cadherin.Results:Compared with the control group,TMP inhibited LLC cells proliferation by dose.After treatment with TMP +DDP for 24h,the invasion and adhesion of LLC-GFP cells were dose-dependently inhibited(P ＜ 0.01).Moreover,TMP +DDP could up-regulate the protein expression of E-cadherin in LLC-GFP cells.Conclusion:TMP + DDP can inhibit the invasion and adhesion of LLC-GFP cells,and the mechanism may be related to up-regulating the protein expression of Ecadherin in LLC-GFP cells.