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为了解三萜类化合物在体内的结合转运机制,采用荧光光谱法结合紫外吸收光谱研究从中药爵床中分离的有效成分木栓酮(Friedelin,FDL)与牛血清白蛋白(Bovine serum albumin,BSA)的结合反应.通过研究木栓酮对牛血清白蛋白内源性荧光的淬灭作用,发现木栓酮可以与牛血清白蛋白相互结合,且其作用是一个静态猝灭的过程.通过计算木栓酮与牛血清白蛋白的结合常数和结合位点,发现在生理弱碱性溶液中二者具有较强的结合作用,且以1:1结合.根据热力学参数推测木栓酮与牛血清白蛋白的作用力类型为氢键和范德华力.根据F rster非辐射能量转移机理,计算出木栓酮与牛血清白蛋白结合距离为2.86 nm.研究表明木栓酮在体内可以通过血清白蛋白进行结合和转运.
To understand the binding and transport mechanism of triterpenoids in vivo, the fluorescence spectra and ultraviolet absorption spectra of Friedelin (FDL) and Bovine serum albumin (BSA) ) By the binding reaction of corticosterone on bovine serum albumin endogenous fluorescence and found that corydosterone and bovine serum albumin binding each other, and its role is a static quenching process by calculating The binding constants and binding sites of xylitol and bovine serum albumin were found to be strongly bound in physiologically weakly alkaline solutions and bound at a ratio of 1: 1. According to the thermodynamic parameters, According to F rster non-radiative energy transfer mechanism, the binding distance between xylitol and bovine serum albumin was calculated to be 2.86 nm. Studies have shown that xylitol can pass through serum albumin Binding and transport.