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目的 通过对 18三体在 NMRI小鼠发生率的研究及 18三体胚鼠和正常胚鼠的大体形态学对照观察 ,进一步认识 18三体 NMRI小鼠的生长发育畸形。方法 6 0只 Han- NMRI母鼠及 11只具有 Rb(2 .18) 6 Rma/ Rb(1.18) 10 Rma染色体结构的雄鼠 ,分别在妊娠第 16和 17天随机分两组断颈处死母鼠 ,6 0只母鼠共有 82 3只子代胚胎着床 ,其中 5 6 9只为活胚。并对所获得的子代活胚进行染色体分析和大体形态学观察。结果 染色体分析发现 5 6 9只活胚中 95只为三体 ,其中 6 8只 18三体小鼠伴有先天性腭裂。 18三体 NMRI小鼠伴有明显的脊柱弯曲 ,此外尚有脐疝、四肢短缩和短颈畸形等少见畸形 ,极个别伴脑外露畸形。结论 18三体 NMRI小鼠的生长发育畸形是一种先天性的发育畸形综合征 ,不仅有体重的发育不足 ,较高的腭裂发生率也是其主要的先天性发育畸形
OBJECTIVE To further understand the growth and deformity of 18-trisomy NMRI mice by studying the incidence of trisomy 18 in NMRI mice and the general morphology control of 18 trisomy and normal embryos. METHODS Sixty male Han-NMRI rats and 11 male Rb (2.18) 6 Rma / Rb (1.18) 10 Rma chromosomes were randomly divided into two groups: A total of 82 3 offspring of 60 rats were implanted, of which 569 were live embryos. Chromosome analysis and gross morphological observation of the resulting live embryos were carried out. Results Chromosome analysis revealed 95 of the 569 live embryos were trisomy, of which 68 were trisomy 18 with congenital cleft palate. 18 trisomal NMRI mice with significant curvature of the spine, in addition there are umbilical hernia, limb shortening and short neck deformities and other rare deformities, a few with brain dew. Conclusion The trisomic NMRI mouse growth and development deformity is a congenital malformation syndrome, not only the development of body weight deficiency, the higher incidence of cleft palate is also its main congenital malformations