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[Objective]To compare the paeoniflorin contents in different parts of Paeonia lactiflora.[Methods]Paeoniflorin contents in different parts of P.lactiflora were detected by HPLC.Chromatographic column was Shim-pack vp-C18(4.6 mm×150 mm,5μm);mobile phase was acetonitrile-0.1%phosphoric acid(14∶86,V/V);detection wavelength was 230 nm;column temperature was 22℃;flow rate was 1.0 mL/min;and injection volume was 20μL.[Results]Paeoniflorin content had good linear relationship with peak area within the range of 6.48-64.80μg/mL.The linear regression equation was Y=12.510X-0.393,R=0.999.The average recovery rate was 98.60%and RSD was 1.23%.Paeoniflorin contents in different parts of P.lactiflora were in the order of rhizomes>roots>leaves>stems>flowers.[Conclusions]This method was simple,rapid,accurate and repeatable,and could be used for the content detection of paeoniflorin in different parts of P.lactiflora.
[Objective] To compare the paeoniflorin contents in different parts of Paeonia lactiflora. [Methods] Paeoniflorin contents in different parts of P. lactiflora were detected by HPLC. Chromatographic column was Shim-pack vp-C18 (4.6 mm × 150 mm, ; detection wavelength was 230 nm; column temperature was 22 ° C .; flow rate was 1.0 mL / min; and injection volume was 20 μL. [Results] Paeoniflorin content had good linear relationship with peak area within the range of 6.48-64.80 μg / mL. The linear regression equation was Y = 12.510X-0.393, R = 0.999. The average recovery rate was 98.60% and RSD was 1.23%. Paeoniflorin contents in Concise] This method was simple, rapid, accurate and repeatable, and could be used for the content detection of paeoniflorin in different parts of P.lactiflora.