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作者用DNA重组技术将多房棘球绦虫特异性抗原的基因片段转入减毒活鼠伤寒沙门氏菌疫苗LT2MIC株中复制表达,以研制能诱发中间宿主和终宿主保护性免疫应答的多价疫苗。将携带多房棘球绦虫基因片段(产生种特异性抗原Ⅱ/3—10)的重组pVM II/3-10质粒,转入鼠伤寒沙门氏菌疫苗LT2MIC株虫,作为重组疫苗;将不含插入基因的pUR278质粒转入鼠伤寒沙门氏菌疫苗LT2MIC
The authors used DNA recombinant technology to transfer the gene fragment of Echinococcus multilocularis specific antigen into an attenuated live Salmonella typhimurium vaccine LT2MIC strain for expression in order to develop a multivalent vaccine that elicits a protective immune response to the intermediate and final hosts. The recombinant plasmid pVM II / 3-10 carrying the Echinococcus multilocularis gene fragment (producing species-specific antigen II / 3-10) was transformed into the Salmonella typhimurium vaccine LT2MIC strain as a recombinant vaccine; Of the pUR278 plasmid into the Salmonella typhimurium vaccine LT2MIC