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基于二硝基苯醚的硫解反应识别机制,设计合成了羟基芘甲醛为荧光基团的探针分子8-(2,4-二硝基苯酚基)芘甲醛(PCNP),研究了PCNP缓冲溶液分散体系对H_2S的响应.未与H_2S作用时,分子内光致电子转移过程导致探针分子PCNP几乎不发光,当体系中存在H_2S时,PCNP发生硫解反应,光致电子转移过程被阻断,羟基芘甲醛发出橙色荧光.PCNP分子对H_2S响应迅速、灵敏,0.1 mmol·L~(-1)硫化氢存在下10 min内荧光强度响应达到最大值,荧光增强达260倍,反应速率常数为0.20 min~(-1),探针分子对H_2S检测限为0.10μmol·L~(-1),并且具有良好选择性.
Based on the recognition mechanism of dinitrophenyl ether for the thiolation reaction, a probe molecule, 8- (2,4-dinitrophenol) pyrene Formaldehyde (PCNP), was designed and synthesized with pyrene as the fluorophore. The effects of PCNP buffer Solution dispersion response to H 2 S. Without the action of H 2 S, intramolecular photo-induced electron transfer leads to almost no emission of probe molecule PCNP. When H 2 S exists in the system, PCNP undergoes thio-reaction and the photo-induced electron transfer process is blocked , The fluorescence of hydroxy pyrene formaldehyde fluoresced orange.PCNP molecule responded quickly and sensitively to H 2 S, the fluorescence intensity reached its maximum within 10 min with 0.1 mmol·L -1 hydrogen sulphide, the fluorescence increased 260 times and the reaction rate constant At 0.20 min -1, the detection limit of H 2 S by probe was 0.10 μmol·L -1, which showed good selectivity.