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目的观察官入菌(米雅 BM)对实验性急性坏死性胰腺炎(ANP)肠道细菌移居的影响。方法 SD 大鼠随机分成三组,对照组、ANP 组和 ANP 米雅BM 处理组,ANP 大鼠模型制备采用胰胆管内注射5%牛磺胆酸钠溶液,观察胰腺及肠道病理形态学改变,血清淀粉酶和脂肪酶活性变化,血清内毒素水平,脏器细菌培养,肠道细菌菌群变化及肠道 sIgA 含量。结果 ANP 组血清淀粉酶、脂肪酶活性为(9091±1901)nkat·L~(-1),(1677±391)nkat·L~(-1),较对照组[(2588±291)nkat·L～(-1),(121±30)nkat·L~(-1)]明显增高,P<0.05;米雅 BM 处理组血清淀粉酶、脂肪酶活性分别为(5528±1195)nkat·L~(-1),(495±129)nkat·L～(-1),较 ANP 组显著下降,P<0.05.米雅 BM 处理组血清内毒素水平为(0.039±O.011)EU·L～(-1),较 ANP 组[(0.067±0.012)Eu·L~(-1)]显著降低,米雅 BM 能显著纠正业已紊乱的肠道菌群,促进肠道有益菌生长,抑制过度繁殖的大肠杆菌等有害菌生长,显著提高肠粘膜双歧杆菌/大肠杆菌(1.96±0.21 vs 1.53±0.31),从而控制肠道细菌移居,脏器细菌培养阳性率显著下降(37.5% vs 80%),肠道 sIgA 含量在 ANP组为(42±18)pg/g,而米雅 BM 处理组[(20±4)pg/g]低于 ANP组,米雅 BM 能显著减轻胰腺及肠道粘膜病理形态损害。结论米雅 BM 可通过调节肠道微生态紊乱,保护肠粘膜屏障,控制 ANP 状态下肠道细菌和毒素移居,从而改善 ANP 肠道及胰腺病理损害。
Objective To observe the effects of organophilic bacteria (Miya BM) on intestinal bacterial colonization in experimental acute necrotizing pancreatitis (ANP). Methods SD rats were randomly divided into three groups: control group, ANP group and ANP Mia BM treatment group. ANP rat model was prepared by intra-pancreatic bile duct injection of 5% sodium taurocholate solution to observe the pancreas and intestinal pathomorphology , Serum amylase and lipase activity changes, serum endotoxin levels, organ culture, changes in intestinal bacterial flora and intestinal sIgA content. Results The levels of serum amylase and lipase in ANP group were (9091 ± 1901) nkat · L -1 and (1677 ± 391) nkat · L -1, respectively, which were significantly higher than that of the control group [(2588 ± 291) nkat · (121 ± 30) nkat · L -1], P <0.05; The activities of amylase and lipase in Mia BM treatment group were (5528 ± 1195) nkat · L ~ (-1) and (495 ± 129) nkat · L ~ (-1), respectively, which were significantly lower than those in ANP group (P <0.05) .The level of endotoxin in Mia BM treated group was (0.039 ± 0.011) EU · L ~ (-1), which was significantly lower than [(0.067 ± 0.012) Eu · L -1] in ANP group. Miya BM could significantly correct the disordered gut microflora, promote the growth of intestinal beneficial bacteria, Bifidobacterium / Escherichia coli (1.96 ± 0.21 vs 1.53 ± 0.31), thus controlling the intestinal bacteria migration, the positive rate of bacterial culture of organ was significantly decreased (37.5% vs 80% ), Intestinal sIgA content in the ANP group was (42 ± 18) pg / g, while the Mia BM treatment group [(20 ± 4) pg / g] was lower than the ANP group, Miya BM can significantly reduce the pancreas and intestine Mucosal pathological damage. Conclusions Mia BM can improve the intestinal and pancreatic pathological changes of ANP by regulating intestinal microflora disorders, protecting the intestinal mucosal barrier and controlling the migration of intestinal bacteria and toxins under ANP.