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利用表面增强拉曼散射(SERS)光谱及激发-发射矩阵(EEM)荧光光谱对食品中非法添加剂苏丹红Ⅰ和合法食品添加剂辣椒红进行了定性分析和检测.SERS光谱结果表明,苏丹红Ⅰ在低波数区域分子的扭转振动信号增强比较明显;而辣椒红在1521和1158 cm-1处拉曼信号增强效果比较明显;EEM荧光光谱结果表明,苏丹红Ⅰ的乙醇溶液在P1(λex=285 nm,λem=345 nm)和P2(λex=335 nm,λem=548 nm)处有2个明显的荧光特征峰;而辣椒红的乙醇溶液有3个特征荧光峰,分别为P1(λex=545 nm,λem=580 nm),P2(λex=560 nm,λem=665 nm)和P3(λex=608 nm,λem=672 nm).
The SERS and EEM fluorescence spectra were used to characterize and detect Sudan Ⅰ, a legal food additive, in pepper. The results of SERS showed that Sudan Ⅰ The torsional vibration signals of low-wavenumber region enhance more obviously. The Raman signal enhancement effect of pepper red at 1521 and 1158 cm-1 is obvious. The results of EEM fluorescence spectroscopy show that the Raman red Ⅰ ethanol solution has the highest enhancement at P1 (λex = 285 nm , λem = 345 nm) and P2 (λex = 335 nm, λem = 548 nm). There were three characteristic fluorescence peaks in capsanthin ethanol solution, which were P1 , λem = 580 nm), P2 (λex = 560 nm, λem = 665 nm) and P3 (λex = 608 nm, λem = 672 nm).