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目的提高早期梅毒诊断的敏感性和特异性。方法采用PCR技术对梅毒螺旋体(Tp)的DNA多聚酶Ⅰ基因(polA)特异性片段进行扩增,共检测385份生殖器溃疡分泌物,同时做暗视野镜检和TpELISA血清学试验。结果 PCR法共检测出阳性75例,敏感性和特异性分别为74.5%、99.3%;PCR法与暗视野镜检及TpELISA结果比较差异有统计学意义。结论 PCR法在早期梅毒诊断中具有快速、有效、结果可靠的优点,可作为血清学的补充试验。
Objective To improve the sensitivity and specificity of early syphilis diagnosis. Methods DNA polymerase Ⅰ gene (polA) fragment of Treponema pallidum (Tp) was amplified by polymerase chain reaction (PCR). A total of 385 samples of genital ulcer secretions were detected. Dark field microscopy and Tp ELISA serological tests were performed simultaneously. Results A total of 75 positive samples were detected by PCR, the sensitivity and specificity were 74.5% and 99.3%, respectively. There was significant difference between PCR and TSPA. Conclusion The PCR method has the advantages of rapid, effective and reliable results in the diagnosis of early syphilis and can be used as a supplementary test of serology.