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目的:观察葡萄内酯对氢溴酸东莨菪碱致大鼠学习记忆障碍的影响,并初步探讨其作用机制。方法:将大鼠随机分为空白组、模型组、阳性药物组以及葡萄内酯低、中、高剂量组,除空白组采用腹腔注射(ip)生理盐水外,其余各组均采用ip东莨菪碱造模,葡萄内酯低、中、高剂量组分别按0.514,0.771,1.028 mg·kg-1灌胃(ig)给药,阳性药物组给予多奈哌齐0.514 mg·kg-1,连续14 d。用Morris水迷宫测定大鼠空间学习记忆能力;并采用紫外分光光度法检测大鼠脑组织中超氧化物歧化酶(SOD),乙酰胆碱酯酶(AChE)的活性以及丙二醛(MDA)和谷氨酸含量。结果:葡萄内酯可以缩短大鼠的逃避潜伏期(P<0.01),葡萄内酯各剂量组可降低AChE活性(P<0.05),其中,葡萄内酯中、高剂量组与模型组比较差异有极显著性(P<0.01);葡萄内酯高剂量组均可降低脑组织中谷氨酸含量(P<0.01);而各剂量组葡萄内酯对SOD活力的提高和MDA含量降低无明显影响。结论:葡萄内酯可以提高对东莨菪碱致学习记忆障碍大鼠的学习记忆能力,其机制可能与和降低AChE活性有关。
Objective: To observe the effect of grape lactone on scopolamine hydrobromide induced learning and memory impairment in rats, and to explore its mechanism. Methods: The rats were randomly divided into blank group, model group, positive drug group and low, middle and high dose of grape lactone group, except for the blank group by intraperitoneal injection of saline, the other groups were used ip scopolamine The model group and the low, middle and high dose groups were administered with ig (0.514,0.771,1.028 mg · kg-1) respectively. The positive group received donepezil 0.514 mg · kg-1 for 14 days. Morris water maze was used to measure the spatial learning and memory ability of rats. The activity of superoxide dismutase (SOD), acetylcholinesterase (AChE) and malondialdehyde (MDA) and glutamine Acid content. Results: VE could shorten the escape latency of rats (P <0.01), and the AChE activity of each dose of VE could be decreased (P <0.05). Among the medium and high dose groups of VE, (P <0.01). The content of glutamate in brain tissue of high-dose group decreased significantly (P <0.01). However, the content of GL had no significant effect on the increase of SOD activity and MDA content in each dose group. Conclusion: Volactone can improve the learning and memory ability of scopolamine-induced learning and memory impairment in rats, and its mechanism may be related to the decrease of AChE activity.