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培养恶性疟原虫培基的上清与不同稀释度的环状体感染红细胞表面抗原(RESA)抗体阳性的10份云南恶性疟患者血样共育,取上清进行RESA-IFA。结果显示,所有血样的RESA抗体滴度均有不同程度的下降.培基经加热处理后,并不影响其抑制活性。表明体外培养恶性疟原虫培基中存在可溶性RESA,且对热稳定。用不同程度的裂殖子可溶性抗原和O型红细胞影抗原与RESA抗体阳性的血样共育,再进行RESA-IFA。裂殖子抗原在浓度为3.1μg/ml时产生完全抑制。相反,O型红细胞影抗原在浓度比裂殖子抗原高约30倍时,才产生抑制。表明RESA源于裂殖子。
The supernatant of cultured P. falciparum culture medium was co-cultured with blood samples from 10 Yunnan falciparum malaria patients positive for different dilution of erythrocyte surface antigen (RESA) antibodies. The supernatant was collected for RESA-IFA. The results showed that all the blood samples of RESA antibody titers have varying degrees of decline.Pei heat treatment, does not affect its inhibitory activity. This indicates that soluble RESA is present in cultured P. falciparum culture medium in vitro and is thermostable. RESA-IFA was co-incubated with RESA antibody-positive blood samples with varying degrees of merozoite soluble antigen and O-type red blood cell antigen. The merozoite antigen produced complete inhibition at a concentration of 3.1 μg / ml. In contrast, O-RBC antigens produce inhibition only at about 30-fold higher concentrations than merozoite antigens. RESA derived from merozoites.