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对红菇深层发酵中起始pH值和发酵时间对红菇菌丝生物量,胞外多糖(EPS)、胞内多糖(IPS)、总多糖(TPS)、最终pH和多糖产率的影响进行了研究,确定了菌丝生长和产物积累的最优化起始pH值和培养时间。研究结果显示:培养条件影响细胞生长和产物合成,培养时间显著影响着菌丝生物量,EPS、IPS、TPS、最终pH和多糖产率;培养基的起始pH显著的影响着EPS、TPS。当培养时间分别为180h、180h、120h、140h时,菌丝生物量、EPS、IPS和TPS分别达到它们的最高点(21.83±0.42)g/L、(1.351±0.117)g/L、(79.87±6.25)mg/g、(2.539±0.187)g/L;当起始pH分别为5.8、4.8、5.8、5.8时,菌丝生物量、EPS、IPS和TPS分别达到它们的最高点(19.34±0.12)g/L、(1.390±0.048)g/L、(1.98±5.06)mg/g、(2.943±0.073)g/L。结果表明:红菇深沉发酵最优化的起始pH值和发酵时间为pH6和140h,胞外多糖形成与菌丝体的生长属于偶联型。这项研究对进一步优化红菇深层发酵高效生产有价值的生物活性代谢产物有着重要的价值。
Effects of initial pH and fermentation time on the mycelia biomass, extracellular polysaccharides (EPS), intracellular polysaccharides (IPS), total polysaccharides (TPS), final pH and polysaccharide yields of Russula submerged fermentation The study determined the optimal initial pH and incubation time for mycelial growth and product accumulation. The results showed that culture conditions affected the cell growth and product synthesis. The culture time significantly affected mycelial biomass, EPS, IPS, TPS, final pH and polysaccharide yield. The initial pH of medium significantly affected EPS and TPS. The mycelium biomass, EPS, IPS and TPS reached their highest point (21.83 ± 0.42) g / L, (1.351 ± 0.117) g / L and (79.87) respectively when the culture time was 180h, 180h, 120h, ± 6.25) mg / g and (2.539 ± 0.187) g / L, respectively. When the initial pH was 5.8, 4.8, 5.8 and 5.8 respectively, the mycelial biomass, EPS, IPS and TPS reached their highest point (19.34 ± 0.12) g / L, (1.390 ± 0.048) g / L, (1.98 ± 5.06) mg / g and (2.943 ± 0.073) g / L respectively. The results showed that the optimal initial pH value and fermentation time of Phragmites australis fermentation were pH6 and 140h. The formation of extracellular polysaccharide and the growth of mycelium belonged to the coupling type. This study has important value for further optimization of deep fermentation of Pleurotus ostreatus and efficient production of valuable bioactive metabolites.