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目前进行逆转录病毒转染细胞多采用静止的方法(static transluction),即将一定浓度的病毒液加入到长有细胞的器皿中,静止放置一定时间。但这种常规方法普遍存在的问题是①转染效率不高,最大转染效率只有58％;②转染所需的病毒上清滴度要高,否则难以达到较高的转染率;③静态转染需有一定浓度的Polybrene存在,否则转染效率极低。
At present, retroviral transfection of cells to use more static method (static transluction), that is, a certain concentration of the virus solution was added to the long cell containers, stand still for a certain period of time. However, the common problems of this conventional method are: (1) the transfection efficiency is not high, the maximum transfection efficiency is only 58%; (2) the virus supernatant titer required for transfection is high, otherwise it is difficult to achieve a high transfection rate; (3) Static transfection requires a certain concentration of Polybrene, or transfection efficiency is extremely low.