紫云英在我国栽培具有悠久历史,目前仍是我国稻区的一种重要绿肥。如何提高其固氮效果,一直是人们关心的问题,接种根瘤菌是一项有效措施,但紫云英老区,土壤中已存在根瘤菌,往往对接种的根瘤菌带来干扰,以致接种效果时有时无,因此,研究根瘤菌彼此间的竞争、侵染结瘤和共生固氮关系,对提高接种效果有重要意义。 一些研究者采用抗药性标记法进行根瘤菌侵染的研究,但这一方法的本身不够完善,常常影响根瘤菌的侵染结瘤和有效性、利用血清学方法对苜蓿、 Astragalus cultivated in our country has a long history, is still an important green manure in China’s rice area. How to improve the effect of nitrogen fixation has always been a matter of concern. Inoculation with Rhizobium is an effective measure. However, Rhizobium has existed in the old area of ​​Ziyunying and has been contaminated with Rhizobium in the soil, resulting in the sometimes inoculation effect sometimes No, therefore, to study rhizobia competition among each other, the relationship between nodulation and nitrogen fixation symbiotic relationship, to improve the vaccination effect is of great significance. Some researchers use the drug resistance marker method for rhizobia infection research, but this method is not perfect enough, often affect the nodulation of nodulation and the effectiveness of infection, the use of serological methods of alfalfa,