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为了探讨Bcl-2和P53蛋白在大肠肿瘤中的表达及与细胞凋亡的关系,用免疫组化方法观察了45例大肠腺瘤和61例大肠癌中Bcl-2和P53蛋白的表达.正常大肠粘膜中Bcl-2和P53均未见表达,而大肠腺癌及大肠癌阳性率均较正常明显增加(P<0.01).大肠腺瘤P53表达随腺瘤大小增加而增加,其中≥20mm组阳性率(77.78%)显著高于<10mm组(35.00%,P<0.05).P53蛋白阳性率也随不典型增生程度增加而增高.P53表达与大肠癌分化程度及Dukes分期有关.大肠癌细胞凋亡指数与Bcl-2阳性表达呈负相关.大肠腺癌中Bcl-2和P53蛋白的表达也呈负相关.Bcl-2蛋白对大肠癌前病变-脉瘤的增殖有一定表达意义,P53在大肠腺瘤癌变和大肠癌进展中起重要作用.它们也是参与细胞凋亡的良好指标.
In order to investigate the expression of Bcl-2 and P53 in colorectal tumors and its relationship with apoptosis, the expression of Bcl-2 and P53 protein in 45 cases of colorectal adenoma and 61 cases of colorectal cancer was examined by immunohistochemistry. There was no expression of Bcl-2 and P53 in colorectal mucosa, but the positive rate of colorectal adenocarcinoma and colorectal cancer was significantly higher than normal (P<0.01). The expression of P53 in colorectal adenoma increased with the size of adenoma, and ≥20mm group. The positive rate (77.78%) was significantly higher than the <10 mm group (35.00%, P<0.05). The positive rate of P53 protein also increased with the increase of atypical hyperplasia. The expression of P53 was related to the degree of differentiation of colorectal carcinoma and Dukes stage. Apoptotic index was negatively correlated with Bcl-2 positive expression. The expression of Bcl-2 and P53 protein was also negatively correlated in colorectal adenocarcinoma. Bcl-2 protein had certain expression significance in the proliferation of precancerous lesion-tumor neoplasm of colorectal carcinoma. P53 It plays an important role in the progression of colorectal cancer and colorectal cancer. They are also good indicators of apoptosis.