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本文通过检测慢性乙型肝炎 (CHB )患者外周血单个核细胞 (PBMC )培养上清中IFN γ、IL 10含量 ,以探讨病毒定量、肝脏炎症病变程度及是否存在HBeAg等因素与细胞因子生成量之间的关系。取CHB患者和健康对照者PBMC ,分别加入特异性刺激剂rHBcAg和非特异刺激剂PHA ,培养 72h后收集上清 ,采用双抗体夹心法检测IL 10和IFN γ。结果表明rHBcAg刺激CHB患者生成的IFN γ与正常对照组无明显差异 ,IL 10较正常对照组高 (P <0 0 1)。病毒量明显影响IFN γ和IL 10的产生 ,IL 10的生成在高病毒量组较高 ,而IFN γ与此相反。按肝脏炎症活动程度分组发现 ,G4、G3组IL 10明显低于G1、G2组 ,但rHBcAg刺激产生的IFN γ在各组间无明显差异。HBeAg ( + )组较HBeAg ( )组生成的IL 10高 ,而IFN γ低。高病毒量、HBeAg抑制CHB患者IFN γ生成 ,而IL 10的生成相对增加 ,肝功能损害程度与IL 10明显负相关。
In this paper, IFNγ and IL-10 in the culture supernatant of peripheral blood mononuclear cells (PBMCs) from patients with chronic hepatitis B (CHB) were detected to explore the relationship between viral quantitative, hepatic inflammatory pathological changes and the existence of HBeAg and cytokine production The relationship between. The PBMCs of CHB patients and healthy controls were taken and rHBcAg and non-specific stimulator PHA were added respectively. The supernatants were collected 72h after culture. IL 10 and IFNγ were detected by double antibody sandwich method. The results showed that IFNγ produced by rHBcAg-stimulated CHB patients had no significant difference from the normal control group, IL 10 was higher than the normal control group (P <0.01). The amount of virus significantly affected the production of IFN [gamma] and IL10, whereas the production of IL10 was higher in the high viral dose group, whereas IFNy was the opposite. According to the degree of hepatic inflammation activity, the levels of IL-10 in G4 and G3 groups were significantly lower than those in G1 and G2 groups. However, there was no significant difference between the groups in the IFNγ production by rHBcAg stimulation. The HBeAg (+) group had higher IL-10 than the HBeAg () group and lower IFN-γ. High viral load, HBeAg inhibited IFNγ production in patients with CHB, while the relative increase in IL 10 production, liver damage and IL 10 was significantly negatively correlated.