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[目的]探讨循环肿瘤细胞(CTC)联合多种血清肿瘤标志物检测对晚期非小细胞肺癌(NSCLC)的诊断价值.[方法]经病理结果确诊的ⅢB期至Ⅳ期NSCLC患者84例作为观察组,肺部良性病变患者60例作为对照组,比较两组外周血CTC、血清癌胚抗原(CEA)、癌抗原125(CA125)、肿瘤相关抗原199(CA199)水平.[结果]观察组患者的血清CTC阳性率、CEA、CA199、CA125水平均显著高于对照组(P<0.05);CTC鉴别诊断NSCLC患者的灵敏度为64.29%、特异度为96.67%,CEA鉴别诊断NSCLC患者的灵敏度为47.62%、特异度为86.67%,CA199鉴别诊断NSCLC患者的灵敏度为36.90%、特异度为76.67%;CTC+CEA+CA125+CA199鉴别诊断NSCLC患者的灵敏度高达88.10%、特异度高达96.67%.[结论]CTC联合多种血清肿瘤标志物检测对晚期NSCLC患者的鉴别诊断能力显著提高.“,”[Objective]To explore the diagnostic value of circulating tumor cells ( CTC) combined with serum tumor markers in patients with advanced non-small cell lung cancer ( NSCLC) . [Methods]A total of 84 patients with pathologi-cal diagnosis of ⅢB ~ⅣNSCLC were selected as the observation subject ( NSCLC group ) . In addition, 60 patients with benign lung lesions served as the control group. MACS negative enrichment joint immunocytochemistry was used to detect peripheral blood CTC. Meanwhile, ECL method usage compared the levels of CTC, serum CEA, CA125 and CA199 in peripheral blood between the two groups. [Results]Generally, the detection levels were significantly higher in the obser-vation group than in the control ( P<0.05) in terms of CTC, CEA, CA199 and CA125 positive rates. Judging by CTC detection, the differential diagnosis of NSCLC patients had a sensitivity of 64.29% and a specificity of 96.67%. CEA dif-ferential diagnosis of NSCLC was 47. 62% in sensitivity and 86. 67% in specificity. Differential diagnosis of NSCLC by CA199 resulted in sensitivity of 36. 90% and specificity of 76. 67%. In the combination of CTC + CEA + CA125 +CA199, the differential diagnosis of NSCLC patients reached sensitivity of 88.10% and specificity of 96.67 %.[Conclu-sion]CTC combined with serum tumor markers for differential diagnosis of patients with advanced NSCLC can be signifi-cantly improved.