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目的:观察妊娠期莪术暴露对大鼠子代发育的影响。方法:Wistar雌性大鼠随机平均分为血瘀证组和正常组,采用皮下注射盐酸肾上腺素及冰水浴复制大鼠血瘀证模型。造模结束后,雌雄2∶1合笼,受孕后正常组和血瘀证组孕鼠随机分为1.4,2.8,5.6 g.kg-1莪术组和对照组。于妊娠6～19 d连续ig给予不同剂量的莪术水煎液。检测仔鼠出牙、开眼、张耳和出毛等生理发育指标,以及平面翻正、断崖回避、负趋地性、前肢悬挂、空中翻正和听觉惊愕等神经行为指标。结果:正常大鼠和血瘀证大鼠给予莪术暴露后两者的负趋地性和断崖回避指标存在差异。在5.6 g.kg-1莪术组,正常大鼠的负趋地性和断崖回避达标日龄负趋地性为(7.00±0.76)d;断崖回避为(5.85±0.81)d,较空白对照组负趋地性为(6.65±0.76)d;断崖回避为(5.45±0.96)d明显延迟(均P<0.05),而血瘀证模型大鼠的仔鼠各项指标与对照组比较无显著性差异。结论:莪术对正常大鼠和血瘀证模型大鼠的神经行为发育的影响具有差异,对正常大鼠的毒性效应更明显。
Objective: To observe the effects of pregnancy during the Curcuma on the development of rats offspring. Methods: Wistar female rats were randomly divided into two groups: blood stasis syndrome group and normal group. Subcutaneous injection of epinephrine hydrochloride and ice water bath were used to duplicate the rat model of blood stasis. After modeling, male and female 2: 1 cage, after conception normal group and blood stasis syndrome pregnant rats were randomly divided into 1.4,2.8,5.6 g.kg-1 Curcuma group and control group. In pregnancy 6 ~ 19 d continuous ig given different doses of zedoary decoction. The physiological and biochemical indexes such as teething, eye opening, ear and hair removal of offspring were detected, as well as neurological behavior indicators such as plane inversion, cliff avoidance, negative geotropism, forelimb suspension, midair turn over and auditory astonishment. Results: There was a difference between the negative geotropism and escarpment avoidance index of normal rats and blood-stasis rats after Curcuma. In the 5.6 g.kg-1 Curcuma group, the negative geotropism of the normal rats and the avoidance standard of the cliffs at 7 days were (7.00 ± 0.76) d and (5.85 ± 0.81) d, respectively, compared with the blank control group Negative geotropism was (6.65 ± 0.76) d; Cliff avoidance was (5.45 ± 0.96) d significantly delayed (all P <0.05), while the blood stasis syndrome rat pups indicators compared with the control group was no significant difference. Conclusion: The effects of Curcuma on the neurobehavioral development of normal rats and blood stasis syndrome rats are different, and the toxic effects on normal rats are more obvious.