论文部分内容阅读
1978～1980年研究了应用玉米小斑病菌T小种毒素粗提液快速测定T型细胞质玉米的技术方法,现报道如下: 材料和方法玉米小斑病菌T小种菌种由全国玉米病害协作组提供。PDA斜面培养冰存。扩大培养用高粱粒煮至半熟,高压灭菌后,接入PDA斜面培养物。置25℃恒温箱培养20～30天。将高粱培养物称重,加2倍蒸馏水,搅碎、浸泡12小时,双层纱布过滤,4000转/分离心沉淀15分钟(或以定性滤纸过滤)。取上清液镜检看不到孢子和菌丝为准。供试玉米材料播于直径10cm瓦盆,留苗3～4株,常规管理到4～5叶期。
From 1978 to 1980, the technical methods for the rapid determination of T-type cytoplasmic maize using the crude extract of T from Bacteroides peltatum were reported as follows: Materials and methods The T. tumefaciens T-minor was cultivated by the National Maize Disease Cooperative Group provide. PDA slant culture ice storage. Expand the cultivation with sorghum grain cook until half-cooked, autoclaved, access PDA slant culture. Set 25 ℃ incubator for 20 to 30 days. Sorghum cultures were weighed, double distilled water, crushed, soaked for 12 hours, double gauze filtered, and centrifuged at 4,000 rpm for 15 minutes (or filtered through a qualitative filter paper). Take the supernatant microscopy spores and mycelium shall prevail. Test corn material sown in 10cm diameter pots, Miao Miao 3 to 4, routine management to 4 to 5 leaf stage.