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本研究旨在建立高脂肪饮食模型,用于评价胡椒酸乙酯的抗高脂血症活性,建立HPLC-MS/MS法测定口服给药后胡椒酸乙酯在大鼠血浆和肝脏中的药代动力学.给大鼠口服施用剂量为50 mg/kg的胡椒酸乙酯化合物.在给药后0.5-10小时通过HPLC检测血浆和肝脏中的胡椒酸乙酯浓度.通过DAS 1.0软件分析药代动力学参数.血浆和肝脏胡椒酸乙酯的浓度-时间曲线符合二室模型.胡椒酸乙酯在胃肠道中迅速被吸收,并且可在口服给药后0.5小时在血浆和肝脏中被检测到.“,”In the present study,we aimed to establish high-fat diet model for evaluating anti-hyperlipidaemic activity of GBA and to establish the HPLC-MS method for measuring the pharmacokinetics of GBA in rat blood plasma and liver after oral administration.Rats were orally administrated with GBA at the dose of 50 mg/kg body weight.GBA concentrations in blood plasma and liver were detected by HPLC from 0.5-10 h post administrations.The pharmacokinetic parameters were analyzed by the DAS 1.0 Software.The concentration-time curves of the plasma GBA and liver GBA were conformed to two-compartment model.GBA was rapidly absorbed in the gastrointestinal tract and could be detected in plasma and liver 0.5 h after oral administration.