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目的 探讨睾丸扭转复位后睾丸组织 NO产生及其变化与睾丸生精功能损伤的关系。方法 用 SD大鼠建立左睾丸扭转复位动物模型 ,复位后用分光光度法分别检测左睾丸组织总抗氧化能力、NO含量和一氧化氮合酶 (NOS)活性变化与睾丸病理改变的关系。结果 睾丸复位后病理损伤明显 ,复位后总抗氧化能力极显著下降 (P<0 .0 1)。NO含量和 NOS活性极显著升高 (P<0 .0 1) ,且两者呈正相关 (rs=0 .85 7)。结论 复位后睾丸组织总抗氧化能力下降、NOS活性升高所致 NO过量产生 ,是造成睾丸扭转复位后病理改变、生精功能损伤的重要原因
Objective To investigate the relationship between the production of testicular tissue NO and the changes of testicular spermatogenic function after testicular torsion and reduction. Methods SD rats were used to establish the animal model of torsion reduction of left testicle. After reduction, the total antioxidant capacity, nitric oxide synthase (NO) content and activity of nitric oxide synthase (NOS) in left testis were detected by spectrophotometry. Results The pathological changes of testis were obvious and the total antioxidant capacity decreased significantly after the reduction (P <0.01). NO content and NOS activity were significantly increased (P <0.01), and the two were positively correlated (rs = 0.857). Conclusion The total antioxidant capacity of testis tissue is decreased and excessive NO production is caused by the increase of NOS activity, which is the important reason for pathological changes and functional impairment of spermatogenesis after testicular torsion reduction