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目的探讨重组人促红细胞生成素(rhEPO)对高浓度葡萄糖(高糖)作用下大鼠视网膜Müller细胞凋亡的保护作用及对核因子-κB(NF-κB)表达的影响。方法将Müller细胞随机分为空白对照组、高糖培养组和不同浓度rhEPO组(分别在高糖培养液中加5、10、20、40kU/L的rhEPO)。培养48h后MTT法检测各组Müller细胞活力,并通过SP法检测rhEPO干预下NF-κB蛋白表达的变化。结果高糖培养组Müller细胞活力较空白对照组显著下降(F=70.39,q=9.13,P<0.01);rhEPO干预后与高糖培养组相比细胞活力明显提高(q=4.30~7.08,P<0.05)。高糖培养组NF-κB表达量较空白对照组明显增高(F=49.76,q=4.37,P<0.05),而各rhEPO组的NF-κB表达量与高糖培养组相比明显增高(q=5.74~27.08,P<0.01)。结论 rhEPO对高糖作用下Müller细胞的凋亡具有保护作用,并能增强NF-κB的表达,其机制可能是通过上调NF-κB的表达来发挥作用。
Objective To investigate the protective effect of recombinant human erythropoietin (rhEPO) on the apoptosis of retinal Müller cells and its effect on the expression of nuclear factor-κB (NF-κB) induced by high concentration of glucose (high glucose). Methods Müller cells were randomly divided into blank control group, high glucose group and rhEPO group (rhEPO at 5, 10, 20, 40 kU / L in high glucose medium). After cultured for 48h, the viability of Müller cells in each group was detected by MTT assay and the expression of NF-κB protein was detected by SP method. Results The viability of Müller cells in high glucose group was significantly lower than that in control group (F = 70.39, q = 9.13, P <0.01). Compared with high glucose group, the viability of Müller cells in high glucose group was significantly increased (q = 4.30-7.08, P <0.05). The expression of NF-κB in high glucose group was significantly higher than that in the blank control group (F = 49.76, q = 4.37, P <0.05), while the expression of NF-κB in rhEPO group was significantly higher than that in the high glucose group = 5.74 ~ 27.08, P <0.01). Conclusion rhEPO can protect the Müller cells from apoptosis induced by high glucose and enhance the expression of NF-κB, which may be related to the up-regulation of NF-κB expression.