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为了观察两株福氏、宋内双价志贺菌苗 (Ipa ,Ipa+ )免疫后所引起的肠粘膜诱导部位的免疫应答及为研制有效腹泻疫苗提供理论基础。应用小鼠灌胃免疫为模型 ,以间接免疫荧光法和BA ELISPOT方法 ,检测了肠粘膜相关淋巴组织 (gutasso ciatedlymphoidtissues ,GALT )中诱导部位派伊尔小结 (Payer’spatches ,PP )、肠系膜淋巴结 (mesentericlymphoidnode ,MLN )T淋巴细胞亚群和特异性抗体分泌细胞的变化 (ASC )及小肠局部和系统的特异性抗体变化。发现两株双价菌苗株均可引起肠粘膜GALT诱导部位CD4+ 淋巴细胞亚群、抗体分泌细胞明显升高 ;同时局部及血清中特异性抗体明显升高。说明双价志贺菌苗灌胃免疫小鼠后 ,诱导部位的免疫应答早期以体液免疫为主 ,灌胃不仅可诱导局部特异性抗体免疫应答也可诱导系统的抗体免疫应答。
In order to observe the immune response induced by two immunized Shigella flexneri and Shigella sonnei (Ipa, Ipa +) in the mucosa of the two Schistosomaks and to provide a theoretical basis for the development of an effective diarrhea vaccine. The gavage immunity of mice was used as a model. Indirect immunofluorescence and BA ELISPOT were used to detect the expression of Payer’s spatches (PP) and mesenteric lymph nodes (GMS) in gutassociated lymphoid tumors (GALT) mesentericlymphoidnode (MLN)), T lymphocyte subsets and specific antibody-secreting cells (ASC), and local and systemic specific antibody changes in the small intestine. It was found that the two strains could induce CD4 + lymphocyte subsets of GALT-induced parts of gut mucosa and the antibody-secreting cells were significantly increased. Meanwhile, the specific antibodies in local and serum were significantly increased. The results showed that the immune response induced by Shigella sonnei was mainly humoral immunity in the early stage after intragastric administration of Shigella flexneri vaccine. Gavage not only induced the local specific antibody but also induced the systemic immune response.