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将分离的牛心肌线粒体先与其ＡＤＰ／ＡＴＰ载体蛋白抑制剂苍术苷交联，然后用ＴｒｉｔｏｎＸ－１００溶解线粒体膜，经羟基磷灰石离心制得该载体蛋白。用聚丙烯酰胺凝胶电泳及生物学方法鉴定，发现所分离的ＡＤＰ／ＡＴＰ载体蛋白分子量约为３０ＫＤ，可作为抗原用于抗该载体蛋白抗体的研究。
The isolated bovine myocardium mitochondria were first crosslinked with its ADP / ATP carrier protein atractyloside, then the mitochondrial membrane was dissolved with Triton X-100 and the carrier protein was obtained by centrifugation of hydroxyapatite. By polyacrylamide gel electrophoresis and biological methods identified, the isolated ADP / ATP carrier protein molecular weight of about 30KD, can be used as an antigen for the study of antibody against the carrier protein.