论文部分内容阅读
以一例重症β＋地中海贫血患儿的脾ＤＮＡ组建了λ噬菌体Ｃｈａｒｏｎ２８基因文库。经β－珠蛋白基因组探针筛选出阳性克隆珠，提取其重组体ＤＮＡ，取其ＢａｍＨＩ／１．８ｋｂ片段，次级克隆至Ｍ１３ｍＰ１８。制备的单链Ｍ１３ｍｐ１８重组体ＤＮＡ经正常与异常的寡核苷酸探针杂交证明该患儿的β地中海贫血基因为β－珠蛋白基因的—２８Ａ→Ｇ突变体。
The λ-phage Charon28 gene library was constructed from spleen DNA from a patient with severe β + thalassemia. The positive clones were screened by β-globin genomic probe and the recombinant DNA was extracted. The BamHI / 1.8kb fragment was obtained and subcloned into M13mP18. The prepared single-chain M13mp18 recombinant DNA was verified by hybridization of normal and abnormal oligonucleotide probes to show that the β-thalassemia gene of this child was -28A → G mutant of β-globin gene.