论文部分内容阅读
目的探讨苯磺酸左旋氨氯地平对腹主动脉部分缩窄大鼠心肌能量代谢的影响及其机制。方法将18只Wistar大鼠随机分为3组:假手术组(Sham组)、手术组(AAC组)和手术+苯磺酸左旋氨氯地平治疗组(AAC+LA组),采用腹主动脉部分缩窄法建立压力超负荷致心肌肥厚的动物模型,AAC+LA组每天给予苯磺酸左旋氨氯地平2.5 mg·kg~(-1)(溶于2 m L的生理盐水中),Sham组及AAC组给予等体积的生理盐水;8周后,测量全心/体质量的比值;颈总动脉插管检测血压;小动物超声测量心脏功能;采用荧光定量(QT-PCR)法检测心肌组织GLUT4 m RNA表达,试剂盒测定心肌组织ADP/ATP比值。结果 8周后与Sham组比较,AAC组血压、全心/体质量比值、左室壁厚度、ADP/ATP比值增加,心脏功能、GLUT4表达下降;AAC+LA组血压、全心/体质量比值、左室壁厚度、ADP/ATP比值下降,心脏功能改善,GLUT4表达上升。结论苯磺酸左旋氨氯地平具有改善压力负荷大鼠心肌收缩、舒张功能的作用,其机制可能与其降低血压的同时增加肥厚心肌GLUT4的表达、改善心肌的能量代谢有关。
Objective To investigate the effect and mechanism of levamlodipine besylate on myocardial energy metabolism in partial aortic constricted rats. Methods Eighteen Wistar rats were randomly divided into three groups: sham group (Sham group), operation group (AAC group) and operation + levamlodipine besylate group (AAC + LA group) The animal models of hypertrophy caused by pressure overload were established by partial narrowing method. AAC + LA group was given levamlodipine besylate 2.5 mg · kg -1 (dissolved in 2 ml saline) daily, Sham The rats in group A and group A were given equal volume of saline. The ratio of total heart to body mass was measured after 8 weeks. The blood pressure was measured by carotid artery cannulation. The cardiac function was measured by ultrasonography in small animals. QT-PCR The expression of GLUT4 m RNA was determined, and the ratio of ADP / ATP in myocardium was determined by kit. Results Compared with Sham group, blood pressure, total heart / body mass ratio, left ventricular wall thickness, ADP / ATP ratio increased and cardiac function and GLUT4 expression decreased in AAC + LA group. Blood pressure and total heart / body mass ratio , Left ventricular wall thickness, ADP / ATP ratio decreased, cardiac function improved, GLUT4 expression increased. Conclusion Levamlodipine besylate can improve myocardial contractile and diastolic function in pressure-overload rats. The mechanism may be related to the decrease of blood pressure and the increase of GLUT4 expression and the improvement of myocardial energy metabolism.