论文部分内容阅读
[目的]研究牛乳碱性蛋白(MBP)对正常、去卵巢雌性大鼠骨密度和股骨中骨Ⅰ型胶原特异性氨基酸含量的影响;分析MBP的氨基酸和蛋白质组成特点。[方法]选取104只雌性SD大鼠,其中48只行卵巢切除手术,12只行假手术,其余44只为正常大鼠。将去卵巢大鼠和正常大鼠分别随机分为4组:对照组、低剂量组、中剂量组和高剂量组。各组相应采用0mg/kg.bw、10mg/kg.bw、20mg/kg.bw、30mg/kg.bw的MBP连续灌胃90d。实验结束收集股骨,并采用双能X线法检测股骨近心端、中段、远心端骨密度,采用自动氨基酸分析仪法检测股骨中羟脯氨酸和脯氨酸的含量。分别采用自动氨基酸分析仪法和SDS-PAGE电泳法分析MBP的氨基酸和蛋白质组成特点。[结果]10mg/kg.bw的MBP能够增加正常大鼠股骨远心端的骨密度;MBP能够提高正常低剂量组和去卵巢中剂量组大鼠股骨中羟脯氨酸和脯氨酸的含量。[结论]MBP对正常大鼠骨密度具有增加作用,但不能逆转去卵巢引起的骨丢失;提高骨Ⅰ型胶原特异性氨基酸含量是其作用于骨骼的重要方式;MBP氨基酸和蛋白质组成特点是其作用于骨骼的基础。
[Objective] To investigate the effect of bovine serum basic protein (MBP) on bone mineral density (BMD) and type I collagen-specific amino acid content in femur in normal and ovariectomized female rats. The amino acid and protein composition of MBP were analyzed. [Method] 104 female SD rats were selected, of which 48 were ovariectomized, 12 were sham operated and the other 44 were normal rats. Ovariectomized rats and normal rats were randomly divided into 4 groups: control group, low dose group, middle dose group and high dose group. Each group corresponding to 0mg / kg.bw, 10mg / kg.bw, 20mg / kg.bw, 30mg / kg.bw MBP continuous gavage 90d. At the end of the experiment, the femurs were collected, and the bone mineral density at the proximal, middle and distal ends of the femur was measured by dual-energy X-ray. The content of hydroxyproline and proline in the femur was detected by automatic amino acid analyzer. The amino acid and protein composition of MBP were analyzed by automatic amino acid analyzer and SDS-PAGE respectively. [Results] MBP at 10 mg / kg.bw increased the bone mineral density at the distal end of femur in normal rats. MBP increased the content of hydroxyproline and proline in the femur of rats in normal low-dose and ovariectomized groups. [Conclusion] MBP can increase bone mineral density in normal rats, but can not reverse the bone loss caused by ovariectomy. Increasing the specific amino acid content of bone collagen type Ⅰ is an important way for bone formation. MBP amino acid and protein composition are characterized by The basis for the role of bone.