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目的:对比分析急性心肌梗死(AMI)患者、造影正常的胸痛患者外周血中Th1,Th2细胞的数量及血浆细胞因子IFN-γ,IL-4水平的差异,从而进一步探讨辅助性T淋巴细胞参与的免疫炎症反应与AMI之间的关系.方法:密度梯度离心法分离来自15例AMI患者、15例造影正常的胸痛患者冠脉内及外周血液中的单个核细胞,细胞荧光染色后用流式细胞仪检测Th1,Th2在血液中的比例,ELISA检测血浆中Th1/Th2分泌的细胞因子IFN-γ,IL-4的含量,分析患者Th1/Th2的变化.结果:AMI患者冠脉阻塞段血液中Th1,IFN-γ含量高于入院时外周血(P<0.05);AMI入院时外周血中Th1,IFN-γ含量高于造影正常的胸痛患者外周血(P<0.05).造影正常的胸痛患者冠脉血Th1,IFN-γ含量与外周血相比无统计学意义.AMI患者冠脉阻塞段血液中Th2,IL-4含量高于入院时外周血(P<0.05).AMI入院时外周血中Th2含量高于造影正常的胸痛患者外周血(P<0.05).AMI入院时外周血中IL-4含量与造影正常的胸痛患者外周血中含量相比无统计学意义;造影正常的胸痛患者冠脉血中Th2,IL-4含量与外周血相比无统计学意义.结论:局部CD4+辅助性T淋巴细胞亚群Th1,Th2的失衡,与AMI和动脉粥样硬化的免疫炎症反应密切相关.
OBJECTIVE: To compare and analyze the difference of Th1 and Th2 cells and the levels of IFN-γ and IL-4 in peripheral blood of patients with acute myocardial infarction (AMI) with normal angiography, so as to further explore the involvement of helper T lymphocytes Immunofluorescence and AMI.Methods: Mononuclear cells from coronary arteries and peripheral blood were collected from 15 AMI patients and 15 normal chest pain patients by density gradient centrifugation. The ratio of Th1 and Th2 in blood was detected by cytometry, the levels of Th1 / Th2 secreted cytokines IFN-γ and IL-4 in plasma were detected by ELISA, and the changes of Th1 / Th2 in patients were analyzed.Results: (P <0.05). The levels of Th1 and IFN-γ in the peripheral blood of AMI patients were higher than those of normal chest pain patients (P <0.05) .Conclusion Normal chest pain The levels of Th1 and IFN-γ in the coronary blood of patients with coronary artery disease were not significantly different from those of the peripheral blood.The levels of Th2 and IL-4 in the coronary occlusion section of AMI patients were higher than those in the peripheral blood at admission (P <0.05) In Th2 content higher than normal angina patients with chest pain (P <0.05) .AMI into The level of IL-4 in peripheral blood was not significantly different from the level in peripheral blood of patients with normal chest pain, and the levels of Th2 and IL-4 in coronary blood with normal angiography were not significantly different from those in peripheral blood. : Local CD4 + T helper lymphocyte subsets Th1, Th2 imbalance, and AMI and atherosclerosis immune inflammation are closely related.