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Rous肉瘤病毒(RSV)、Abelson肉瘤病毒和Harvey肉瘤病毒(HaSV)对细胞转化都受一条病毒多肽的调节,RSV转化蛋白p60~(src)是对酪氨酸残基有严格特异性的蛋白激酶,Abelson病毒的转化蛋白P120对含酪氨酸蛋白有激酶活性,HaSV的转化基因编码一条多肽p21,它对鸟嘌呤核苷酸有惊人的亲和力。这些有激酶活性的转化蛋白对细胞成份多肽的磷酸化可能在细胞被病毒感染后的恶性转化中起重要作用。作者用免疫荧光、免疫电镜和细胞分级离析等方法研究指出p60~(src)及其底物广泛分
Rous sarcoma virus (RSV), Abelson sarcoma virus and Harvey sarcoma virus (HaSV) are both regulated by a viral polypeptide, and the RSV transforming protein p60 ~ (src) is a protein kinase with strict specificity for tyrosine residues , The Abelson virus’s transforming protein P120 has kinase activity on tyrosine-containing proteins and the HaSV transgene encodes a polypeptide, p21, which has a surprising affinity for guanosine nucleotides. The phosphorylation of these cellular components with these kinase-active transforming proteins may play an important role in the malignant transformation of cells after being infected by the virus. The authors used immunofluorescence, immunoelectron microscopy and cell fractionation studies have shown that p60 ~ (src) and its substrate is broadly divided