利用甲基磺酸乙酯(ethylmethane sulphonate,EMS)诱变粳稻品种日本晴获得了一个遗传稳定的叶形突变体thread-like leaf1(tll1)。该突变体在杭州表现为矮化、窄叶,极端时仅剩主脉,呈细丝状。将该突变体分别与籼稻品种南京6号、浙辐802和9311进行正反交配组,遗传分析表明该突变体性状由1对隐性单基因控制。通过SSR和STS分子标记对F2代分离群体进行遗传定位,将该基因初步定位在第12染色体SSR标记RM247和RM101之间。随后利用已公布的粳稻品种日本晴和籼稻品种9311的基因组序列,发展了7对有多态的STS标记,最终将该基因定位在FL13和FL14之间约94.3kb的区间内,为进一步克隆TLL1基因奠定了基础。 A genetically stable leaf-like leaf mutant (thread-like leaf1 (tll1)) was obtained by inoculation of Japonica rice with ethylmethane sulphonate (EMS). The mutant in Hangzhou showed dwarf, narrow leaves, only the main pulse at the extreme, was filamentous. The mutant was crossed with the indica rice Nanjing 6, Zhefu 802 and 9311, respectively. The genetic analysis indicated that the mutant trait was controlled by a single recessive single gene. The SSR and STS molecular markers were used to locate F2 segregation population and the gene was initially located between SSR markers RM247 and RM101 on chromosome 12. Seven pairs of polymorphic STS markers were then developed using the published Japanese genomic sequence Nipponbare and indica variety 9311. The final STS marker was located within a range of about 94.3 kb between FL13 and FL14. To further clone the TLL1 gene Foundation.