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HBV前—s基因编码的蛋白是包裹病毒的组成成分,在血清中它们和高度病毒复制标志一起被发现。但关于他们在肝脏内时HBV复制状态是处于高度还是低度则知之甚少。本文作者报告慢性HBV感染过程中肝脏内前-s编码蛋白的存在和细胞定位及它们与血清病毒标志表达的相互关系进行了比较。材料和方法本研究30例病人(男27,女3,平均年龄44.3岁),他们患有慢性HBV感染和不同严重程度的肝脏病,无delta肝炎病毒(HDV)双重感染。5例HBeAg阳性,23例抗-HBe阳性,2例两种标志皆阴性。从所有这些病人取新鲜冰冻肝活检标本,经恒冷切片机切片,对HBV基因组肽的密码以及对HBsAg、HBcAg、HDAg用单克隆和多克隆抗体进行直接和间接免疫
The proteins encoded by the HBV pre-s gene are part of the virus that surrounds the virus and are found in the serum along with highly viral replication markers. Little is known about whether HBV replication status is high or low in the liver. The authors report the presence and location of pre-s-encoded proteins in the liver during chronic HBV infection and their correlation with serum viral markers. MATERIALS AND METHODS: Thirty patients (27 males, 3 females, mean age 44.3 years) with chronic HBV infection and varying degrees of liver disease and no dual hepatitis delta virus (HDV) infection were studied in this study. 5 cases of HBeAg-positive, 23 cases of anti-HBe positive, two cases of two signs were negative. Fresh frozen liver biopsies were taken from all of these patients and cryogenically and directly immunized with cryogenic microtome sections, the codons for HBV genomic peptides, and the monoclonal and polyclonal antibodies to HBsAg, HBcAg, and HDAg