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作者们用VeroE-6细胞培养观察了声频范围交变磁场对细胞抗病毒防御作用的影响.VeroE-6细胞在加青霉素营养基中培育1～2天形成单细胞层,用磁感强度0.1～400μT 的2000Hz 交变磁场处理5～90min后,除去培养液,用液清洗单细胞层后,接种50%组织细胞染毒量(61g/0.2ml)的西尼罗河热病毒,37℃孵化1～1.5小时,除去未被吸收的病毒液,用液清洗2遍。加入无血清培养液于3,6
The authors used VeroE-6 cell culture to observe the effect of the alternating magnetic field in the audible frequency range on the antiviral defense of cells.VeroE-6 cells were cultured in penicillin-supplemented medium for 1 to 2 days to form a single cell layer with magnetic induction of 0.1 ~ 400μT 2000Hz alternating magnetic field treatment 5 ~ 90min, the culture medium was removed, the liquid monolayer was washed with liquid, inoculated with 50% of the tissue cells exposed to the amount (61g / 0.2ml) of West Nile virus, incubated at 37 ℃ 1 ~ 1.5 Hours, remove the virus liquid is not absorbed, with liquid wash 2 times. Add serum-free medium at 3,6