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[目的]探讨阿魏酸钠(SF)对氧化低密度脂蛋白(ox-LDL)诱导人脐静脉内皮细胞株(HUVEC-CRL1730)凋亡的抑制作用及可能机制。[方法]体外ox-LDL诱导人脐静脉内皮细胞损伤,用阿魏酸钠进行干预。采用四甲基偶氮唑盐法检测细胞存活率;Hoechst33258细胞核荧光染色法观察细胞凋亡的形态学变化;流式细胞术检测细胞凋亡率;Western-blot检测内皮细胞p53蛋白表达。[结果]ox-LDL可明显抑制人脐静脉内皮细胞生长和增殖,诱导细胞发生凋亡,上调p53蛋白表达水平;阿魏酸钠干预后细胞存活率明显上升(P﹤0.05),细胞凋亡率明显下降(P﹤0.05),p53蛋白表达水平明显下调(P﹤0.05)。[结论]ox-LDL可通过上调p53蛋白表达诱导人脐静脉内皮细胞凋亡,阿魏酸钠可通过下调p53蛋白表达抑制内皮细胞凋亡。
[Objective] To investigate the inhibitory effect of sodium ferulate on the apoptosis of human umbilical vein endothelial cells (HUVEC-CRL1730) induced by ox-LDL and its possible mechanism. [Method] Ox-LDL induced the injury of human umbilical vein endothelial cells in vitro and intervention with sodium ferulate. The cell viability was detected by MTT assay. Morphological changes of apoptosis were observed by Hoechst33258 staining. Flow cytometry was used to detect the apoptosis rate. Western blot was used to detect the expression of p53 protein in endothelial cells. [Results] Ox-LDL could significantly inhibit the growth and proliferation of human umbilical vein endothelial cells, induce cell apoptosis and up-regulate the expression of p53 protein. Cell survival rate was significantly increased after intervention with sodium ferulate (P <0.05) (P <0.05), and the expression of p53 protein was significantly down-regulated (P <0.05). [Conclusion] Ox-LDL can induce the apoptosis of human umbilical vein endothelial cells by up-regulating the expression of p53 protein. Sodium ferulate inhibits the apoptosis of endothelial cells by down-regulating the expression of p53 protein.