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目的 :观察瘦素受体 (LR)在条件性厌味 (CTA)大鼠脑的表达 .方法 :采用高度特异的抗瘦素受体血清 ,用免疫组化ABC法观察LR在CTA和对照大鼠脑的分布与表达 .结果 :与对照组大鼠相比 ,CTA大鼠皮层Ⅲ层、视交叉上核、弓状核、杏仁基底外侧核、海马CA3、下丘脑外侧区等核团瘦素受体表达增加 (14 6vs 12 0 ,138vs 92 ,118vs 78,10 7vs70 ,85vs 38cells·mm-2 ,P <0 .0 5 ) ,而下丘脑前区、脑桥臂旁核、杏仁中央核瘦素受体表达减弱 (13vs2 6 ,70vs5 5 ,12 2vs 16 2cells·mm-2 ,P <0 .0 5 ) ,孤束核瘦素受体的表达与对照大鼠无显著性差异 (5 0vs 5 7cells·mm-2 ,P >0 .0 5 ) .结论 :瘦素可能通过杏仁核、臂旁核、海马、皮层的瘦素受体参与CTA的形成与维持
OBJECTIVE: To observe the expression of leptin receptor (LR) in the brain of conditional unpleasant taste (CTA) rats.Methods: Using highly specific anti-leptin receptor serum, the expression of LR in CTA and control RESULTS: Compared with the control group, the levels of cortical L3, the suprachiasmatic nucleus, the arcuate nucleus, the apical lateral nucleus of the almond, the hippocampal CA3, the hypothalamic lateral leptin nucleus leptin (14 6 vs 12 0, 138 vs 92, 118 vs 78, 10 7 vs 70, 85 vs 38 cells · mm -2, P <0.05), whereas the hypothalamic precordial, parabrachial nucleus, amygdala central leptin (13vs2 6, 70vs5 5, 12 2vs 16 2cells · mm-2, P <0.05). There was no significant difference in the expression of leptin receptor between nucleus tractus solitarius and control rats (50 vs 5 7 cells · Mm-2, P> 0.05) .Conclusion: Leptin may participate in the formation and maintenance of CTA through the amygdala, parabrachial nucleus, hippocampus and cortical leptin receptors