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目的：观察实验兔急性肺损伤中血管内皮标记物的变化。方法：采用同种骨髓提取液（ＢＭＥ）经兔颈静脉缓慢恒流注入致伤的方法，复制兔骨髓型急性肺损伤（ＡＬＩ）模型，连续６ｈ多时相点观察血中循环内皮细胞（ＣＥＣ）、血浆颗粒膜蛋白－１４０（ＧＭＰ－１４０）和内皮素（ＥＴ－１）以及肺组织ＧＭＰ－１４０含量，并行常规病理及电镜检查。结果：致伤后０．５ｈ，ＣＥＣ、血浆ＧＭＰ－１４０和ＥＴ－１均显著升高，持续６ｈ仍显著高于伤前和对照；其中，血浆ＧＭＰ－１４０升高达３．２５倍，１ｈ时达到高峰，而肺组织ＧＭＰ－１４０含量降低。血浆ＧＭＰ－１４０早期升高，幅度较大，与ＰａＯ２负相关，与其它指标亦有较好的相关性。结论：经兔颈静脉缓慢恒流速注射ＢＭＥ（０．３５ｍｌ／ｋｇ，２ｍｌ／ｈ）能复制出较理想的ＡＬＩ模型。检测血浆ＧＭＰ－１４０可能为临床ＡＬＩ的早期诊断、病情监测和评估提供依据。
Objective: To observe the changes of vascular endothelial markers in experimental acute lung injury in rabbits. Methods: Rabbit bone marrow-derived acute lung injury (ALI) model was induced by slow and constant current injection of rabbit bone marrow using the same bone marrow extract (BME). The levels of circulating endothelial cells (CECs) , Plasma membrane protein-140 (GMP-140) and endothelin (ET-1) and lung tissue GMP-140 content, routine pathology and electron microscopy. Results: At 0.5h after injury, the levels of CEC, plasma GMP-140 and ET-1 were significantly increased, and remained significantly higher at 6h than pre-injury and control. The plasma GMP-140 level was 3.25 folds higher at 1h Peaked, while lung tissue GMP-140 levels decreased. Plasma GMP-140 increased early, with a greater magnitude, negative correlation with PaO2, and other indicators also have a good correlation. CONCLUSION: BME (0.35ml / kg, 2ml / h) injected slowly and slowly through rabbit’s jugular vein can replicate the more ideal ALI model. Detection of plasma GMP-140 may provide the basis for the early diagnosis, disease monitoring and evaluation of clinical ALI.