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我们目前做细菌培养基主要应用胎盘,制做简便并且经济。茲将其做法介绍如下:一、取新鲜胎盘,洗去外面之血及粘液,剪去脐带和羊膜,放入碎肉器内(或研钵内)捣碎,称其重量按1∶5之比,加入生理盐水制成混合液,静置2-4小时(并加摇动),然後置入高压滅菌器内15-17磅之压力下,消毒30-40分钟。二、取出冷却、过滤後,校正其PH(氢游子)使成为7.2-7.4,即可代替肉汤使用,若做平皿或斜面培地,只用此液100c.c.加入2gm的瓊脂(Agar-Agar)即可。三、做各种糖发酵用,可在制好的胎盘液内(每100c.c.)加入1gm的蛋白腖再须校正一下PH,
We currently use bacterial culture medium mainly for the application of placenta, making it simple and economical. Here’s how it will be described as follows: 1. Take fresh placenta, wash out the blood and mucus, cut the umbilical cord and amniotic membrane, put it in the mincer (or in the mortar), mash it, and call it a weight of 1:5. For comparison, a mixture of physiological saline was added to make a mixture, allowed to stand for 2-4 hours (with shaking), placed in an autoclave at a pressure of 15-17 pounds, and sterilized for 30-40 minutes. Second, take out the cooling, filtration, correction of its PH (hydrogen) so that it becomes 7.2-7.4, can be used instead of broth, if do petri dishes or slant cultivation, only use this liquid 100c.c. add 2gm of agar (Agar- Agar) Third, do all kinds of sugar fermentation, can be added in the prepared placenta solution (per 100c.c.) 1gm peptone must be corrected PH,