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目的调查北京地区冬季肠道门诊就诊腹泻患者人杯状病毒的感染情况。方法收集北京市2007年12月至2008年3月12家医院肠道门诊就诊腹泻患者271例,采集患者粪便标本,使用反转录-聚合酶链反应(RT-PCR)对粪便标本进行人杯状病毒RNA检测,对RT-PCR阳性标本的PCR产物进行克隆测序。结果检测271例粪便标本,共有87例阳性(87/271,32.10%)。选择其中21例PCR产物进行克隆测序,将获得的序列进行比对分析、构建系统发生树,结果表明,21株全部为诺如病毒,未见扎如病毒。其中GⅡ/4型16株(16/21,76.19%),GⅡ/6型1株(1/21,4.76%),GⅡ/7型1株(1/21,4.76%),GⅡ组未定型3株(3/21,14.29%)。结论北京地区冬季肠道门诊就诊的腹泻患者人杯状病毒感染较高,主要流行株为诺如病毒GⅡ/4型。
Objective To investigate the status of human calicivirus infection in diarrhea patients in winter gut clinics in Beijing. Methods Totally 271 patients with diarrhea were collected from 12 clinics in Beijing from December 2007 to March 2008, and the stool samples of patients were collected. The stool samples were collected by RT-PCR Like virus RNA detection, PCR products of RT-PCR positive specimens were cloned and sequenced. Results 271 stool specimens were detected, of which 87 were positive (87/271, 32.10%). 21 of the PCR products were selected for cloning and sequencing, and the obtained sequences were compared and the phylogenetic tree was constructed. The results showed that all 21 strains were Norovirus and no Zarvirus was found. Among them, 16 (21/21, 76.19%) of GⅡ / 4, 1 of GⅡ / 6 (1 / 21,4.76%) and 1 of GⅡ / 7 (1 / 21,4.76% 3 strains (3/21, 14.29%). Conclusions The incidence of calicivirus infection is higher in patients with diarrhea in Beijing winter gout clinic. The main epidemic strain is Norovirus GⅡ / 4.