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目的探讨乌司他丁(Ulinastatin,UTI)和泰索帝(Taxotere,TXT)对体外培养的人乳腺癌细胞MDA-MB-231中u-PA、uPAR、ERK表达的影响。方法将MDA-MB-231(ER-)细胞分为4组:UTI组(UTI 800 U/ml)、TXT组(TXT 3.7μg/ml)、UTI+TXT组(UTI 800 U/ml+TXT 3.7μg/ml)、对照组(等量生理盐水)。给药后24 h,分别采用荧光定量RT-PCR检测各组细胞中uPA、uPAR、ERK基因mRNA的水平,Western blot法检测各组细胞中uPA、uPAR、p-ERK1/2蛋白的表达水平。结果 UTI组和UTI+TXT组MDA-MB-231(ER-)细胞中uPA和uPAR基因mRNA的水平均明显低于对照组(P<0.05),而TXT组中二者的表达水平均明显高于对照组(P<0.01),各组间ERK基因mRNA的水平差异无统计学意义(P>0.05);UTI组和UTI+TXT组中uPA、uPAR和p-ERK1/2蛋白的表达水平均明显低于对照组(P<0.01),而TXT组中3种蛋白的表达水平均明显高于对照组(P<0.05)。结论UTI可抑制MDA-MB-231细胞中uPA、uPAR、p-ERK的表达,而TXT可上调三者的表达。
Objective To investigate the effects of Ulinastatin (UTI) and Taxotere (TXT) on the expression of u-PA, uPAR and ERK in human breast cancer cell line MDA-MB-231 cultured in vitro. Methods UTI group (UTI 800 U / ml), TXT group (TXT 3.7 μg / ml) and UTI + TXT group (UTI 800 U / ml + TXT 3.7 μg / ml), control group (equal amount of saline). The expression of uPA, uPAR and ERK mRNA was detected by RT-PCR at 24 h after treatment. The expressions of uPA, uPAR and p-ERK1 / 2 in each group were detected by Western blot. Results The mRNA levels of uPA and uPAR in MDA-MB-231 (ER-) cells in UTI group and UTI + TXT group were significantly lower than those in control group (P <0.05), while those in TXT group were significantly higher There was no significant difference in ERK mRNA levels between the two groups (P> 0.05). The expression levels of uPA, uPAR and p-ERK1 / 2 in UTI group and UTI + TXT group were significantly lower than those in control group (P <0.01), while the expression levels of three proteins in TXT group were significantly higher than those in control group (P <0.05). Conclusion UTI can inhibit the expression of uPA, uPAR and p-ERK in MDA-MB-231 cells, while TXT can up-regulate the expression of all three.